OSD-411 · GLDS-411
Plant Gravity Perception: Pilot (PGP Pilot)
Study overview
What the record says
This study examined tissue-specific early transcriptional responses upon reorientation in wild type and starchless pgm-1 mutant seedlings of Arabidopsis. Seedlings were grown in vertical Petri dishes for four days. Treatment samples were reoriented 90 degrees for ten minutes while control plants remained vertical. All seedlings were flash frozen in liquid nitrogen and RNA was extracted from root tips, mature root, hypocotyl, and cotyledon fractions of the seedlings. RNA was sequenced via NovaSeq.
Structured metadata
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01
Biological subject
- Organism
- Arabidopsis thaliana
- Material
- root tipCotyledonmature rootHypocotyl
02
Measurement
- Assay
- RNA Sequencing (RNA-Seq)
- Measures
- transcription profiling
- Platform
- Illumina
03
Experimental factors
- Factor type
- Genotype Organism Part treatment
- Factor name
- Genotype, Organism Part, Treatment
04
Mission context
- Space program
- NASA
People & publication
Follow the work
- Investigators
- Chris Wolverton
- Managing center
- Kennedy Space Center (KSC)