OSD-532 · GLDS-532
Space flight mouse plasma cell-free RNA sequencing in Mouse Habitat Unit-1 (MHU-1)
Study overview
What the record says
Purpose: Sequencing analysis of plasma cell-free RNA was performed to study the effects of space flight and microgravity environment in mice. Methods: Plasma samples were collected during the JAXA MHU-1 mission as reported previously (Shiba et al., 2017, Scientific Reports). Total RNA was purified from plasma samples and processed for sequencing analysis, to compare RNA expression profiles in ground control, space flight and artificial 1-G control groups.
Structured metadata
Map the experiment
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01
Biological subject
- Organism
- Mus musculus
- Material
- Plasma
02
Measurement
- Assay
- RNA Sequencing (RNA-Seq)
- Measures
- transcription profiling
- Platform
- Illumina
03
Experimental factors
- Factor type
- Space Flight Gravity Altered
- Factor name
- Spaceflight, Altered Gravity
04
Mission context
- Mission
- SpaceX-9
- Flight program
- International Space Station (ISS)
- Space program
- JAXA
People & publication
Follow the work
- Publication
- Release of CD36-associated cell-free mitochondrial DNA and RNA as a hallmark of space environment response
- Investigators
- Masafumi Muratani