Sex‐Specific Changes in Brain Organoids from Acute Simulated Galactic Cosmic Radiation are Rescued by Targeted ECM Pathway Stimulation (sex-specific response experiment)

This study aims to characterize transcriptomic responses to Simplified 5 Ion GCR Simulation (simGCRsim) in human brain organoids derived from hiPSCs. Two independent RNA-seq experiments were conducted. The first set compared male- and female-derived organoids to evaluate sex-specific transcriptional responses to GCR. The second set assessed organoids derived from i) male hiPSCs expressing optogenetically-controlled FGFR (opto-FGFR) or mock constructs to explore FGFR signaling as a potential countermeasure and ii) one female hiPSC line (SC2131), with or without GCR exposure. Together, these datasets provide insight into both intrinsic sex differences and FGFR-dependent protective mechanisms in human neural tissues in the context of space radiation exposure. This study host the data of the sex-specific response experiment, and the data of Opto-FGFR-mediated rescue under simGCRsim exposure is available in OSD-1000.